Journal: Molecules

Article Title: Hypoxia-Driven Functional Conversion of CAPE: From Anti-Inflammatory to Pro-Tumorigenic Action in the Human Astrocytoma Cell Line CCF-SSTG1

doi: 10.3390/molecules31010140

Figure Lengend Snippet: Viability of CCF-STTG1 cells treated with CAPE, LPS, and IFN-α under normoxia (white boxes) and hypoxia (gray boxes) evaluated by the MTT assay ( n = 3). Results are expressed as percentage of control viability (mean ± SD). The box plots show the median (horizontal line), mean (□ symbol), and interquartile range (IQR). Whiskers represent the range of values excluding outliers.

Article Snippet: Lipopolysaccharide (LPS; E. coli O26:B6) was obtained from Difco Laboratories (Detroit, MI, USA).

Techniques: MTT Assay, Control

Journal: Molecules

Article Title: Hypoxia-Driven Functional Conversion of CAPE: From Anti-Inflammatory to Pro-Tumorigenic Action in the Human Astrocytoma Cell Line CCF-SSTG1

doi: 10.3390/molecules31010140

Figure Lengend Snippet: The effect of CAPE on selected cytokines: IL-8, IL-10, IL-26, metalloproteinases MMP-1, -2, -3 and PTX3 by native and stimulated astrocytes cell line CCF-STTG1 by LPS or/and IFN-α in normoxic (left figures) and hypoxic (right figures) conditions. Data are expressed as mean ± SD (pg/mL) derived from three independent biological replicates (n = 3). Boxes represent the interquartile range (IQR), with the horizontal line indicating the median and the ‘x’ marking the mean. Whiskers show the minimum and maximum values (excluding outliers). Green and orange colors denote data from normoxic and hypoxic conditions, respectively.

Article Snippet: Lipopolysaccharide (LPS; E. coli O26:B6) was obtained from Difco Laboratories (Detroit, MI, USA).

Techniques: Derivative Assay

Journal: Applied Sciences

Article Title: The Impact of Sea Buckthorn (Hippophae rhamnoides L.) and Cornelian Cherry (Cornus mas L.) Plant Extracts on the Physiology of Gastrointestinal Tract Cell In Vitro Model in the Context of Metabolic Diseases

doi: 10.3390/app14177992

Figure Lengend Snippet: Figure 5. Reactive oxygen production (oxidative burst) in PMA-stimulated Raw 264.7 cells (a–d). The plots show luminescent signals using arbitrary units expressed as a percentage of the value of the control at the start of the reaction (time 0), the same letters represent lack of statistically significant differences at p < 0.05; ns—insignificant differences. (e) Concentration of NO metabolites (nitrites nmol/(ml × cell)) in the culture supernatant from P388D1 monocytes stimulated with LPS. (f) Concentration of IL-1β in the culture supernatant from P388D1 monocytes stimulated with LPS. * p < 0.05 vs. Con. RL—sea buckthorn extract (10, 50 µg/mL); RO—sea buckthorn fruit extract (10, 50 µg/mL); DL—Cornelian cherry leaf extract (10, 50 µg/mL); DO—Cornelian cherry fruit extract (10, 50 µg/mL); Con—cells treated with the appropriate amount of solvent. Panels (e,f) created with https://www.biorender.com/.

Article Snippet: Next, the cells were stimulated with 100 ng/mL lipopolysaccharide (LPS from E. coli O26:B6, Merck Life Sciences, USA) for 30 min. A suspension of oxidised LDL from human plasma (Thermo Scientific, USA, #L34357) in the final concentration of 50 μg/mL was then added for 90 min.

Techniques: Control, Concentration Assay, Solvent

Journal: iScience

Article Title: Endoplasmic reticulum expansion throughout the differentiation of teleost B cells to plasmablasts

doi: 10.1016/j.isci.2022.105854

Figure Lengend Snippet:

Article Snippet: Lipopolysaccharides from Escherichia coli O26:B6 , Merck Millipore , Cat#L8274.

Techniques: Immunopeptidomics, Recombinant, Labeling, Conjugation Assay, Staining, Protease Assay, Fluorescence, Saline, SYBR Green Assay, Electron Microscopy, Real-time Polymerase Chain Reaction, Software

Journal: Frontiers in Cellular Neuroscience

Article Title: Application of an in vitro neuroinflammation model to evaluate the efficacy of magnesium-lithium alloys

doi: 10.3389/fncel.2024.1485427

Figure Lengend Snippet: Characterization of D'U937 response to LPS+IFNγ stimulation. U937 monocytes were differentiated to macrophages and treated with 10 ng/mL LPS and 5 ng/mL IFNγ for 24 h. (A) Gene expression changes analyzed by a qPCR array. One array plate was used for the untreated cells and one plate for the LPS-stimulated cells. (B) Cytokine concentrations in the conditioned media measured by ELISA. Graph shows mean ± SD for results from assays (2 technical replicates per sample per assay) performed on three different aliquots of the conditioned media.

Article Snippet: The U937 (ATCC CRL-1593.2, procured from Deutsche Sammlung von Mikroorganismen und Zellkulturen, Germany) cells were grown until a density of 500,000 cells/mL in RPMI-1640 medium (R0883, Merck, Germany) supplemented with 10% FBS, 2 mM L-glutamine (25030081), 10 mM HEPES (15630080) (both Thermo Fisher, Germany), and 1 mM sodium pyruvate (P5280, Merck, Germany) and differentiated to macrophages (hence called D'U937) by exposing them 10 ng/mL phorbol 12-myristate 13-acetate for 48 h. After differentiation, the medium was replaced with MEMα+10% FBS and the cells were allowed to adapt for 24 h. D'U937 cells were treated with 10 ng/mL bacterial lipopolysaccharide (LPS, E. coli O26:B6, L2654, Merck, Germany) together with 5 ng/mL interferon gamma (IFNγ, 11343534, ImmunoTools, Germany) for 24 h. Then, this conditioned medium (henceforth called LPSCM) was sterile filtered and stored at −80°C until use.

Techniques: Gene Expression, Enzyme-linked Immunosorbent Assay